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- Title
- Flavonoid compound-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris
- Publication Year
- 2012-05-01
- Abstract
- The3C-likeprotease(3CLpro)ofsevereacuterespiratorysyndromeassociatedcoronavirus(SARS-CoV)isvitalforSARS-CoVreplicationandisapromisingdrugtarget.Recombinant3CLprowasexpressedinPichiapastorisGS115asa42kDaproteinthatdisplayedaKmof152withDabcyl-KTSAVLQSGFRKME-Edansassubstrate.Purified3CLprowasusedforinhibitionandkineticassayswithsevenflavonoidcompounds.TheIC50ofsixflavonoidcompoundswere4781.Quercetin,epigallocatechingallateandgallocatechingallate(GCG)displayedgoodinhibitiontoward3CLprowithIC50valuesof73,73and47,respectively.GCGshowedacompetitiveinhibitionpatternwithKivalueof251.7.Inmoleculardockingexperiments,GCGdisplayedabindingenergyof?14kcalmol?1totheactivesiteof3CLproandthegalloylmoietyat3-OHpositionwasrequiredfor3CLproinhibitionactivity.
- Keyword
- Flavonoid; Moleculardocking; Pichiapastoris; Protease; Severeacuterespiratorysyndrome(SARS)
- Journal Title
- Biotechnologyletters
- Citation Volume
- 34
- ISSN
- 0141-5492
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
- 7. KISTI 연구성과 > 학술지 발표논문
