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- dc.contributor.author
- 티탄항응우엔
- dc.contributor.author
- 안설아
- dc.date.accessioned
- 2019-08-28T07:41:14Z
- dc.date.available
- 2019-08-28T07:41:14Z
- dc.date.issued
- 2012-05-01
- dc.identifier.issn
- 0141-5492
- dc.identifier.uri
- https://repository.kisti.re.kr/handle/10580/14097
- dc.identifier.uri
- http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART62436871
- dc.description.abstract
- The3C-likeprotease(3CLpro)ofsevereacuterespiratorysyndromeassociatedcoronavirus(SARS-CoV)isvitalforSARS-CoVreplicationandisapromisingdrugtarget.Recombinant3CLprowasexpressedinPichiapastorisGS115asa42kDaproteinthatdisplayedaKmof152withDabcyl-KTSAVLQSGFRKME-Edansassubstrate.Purified3CLprowasusedforinhibitionandkineticassayswithsevenflavonoidcompounds.TheIC50ofsixflavonoidcompoundswere4781.Quercetin,epigallocatechingallateandgallocatechingallate(GCG)displayedgoodinhibitiontoward3CLprowithIC50valuesof73,73and47,respectively.GCGshowedacompetitiveinhibitionpatternwithKivalueof251.7.Inmoleculardockingexperiments,GCGdisplayedabindingenergyof?14kcalmol?1totheactivesiteof3CLproandthegalloylmoietyat3-OHpositionwasrequiredfor3CLproinhibitionactivity.
- dc.language
- eng
- dc.relation.ispartofseries
- Biotechnologyletters
- dc.title
- Flavonoid compound-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris
- dc.citation.endPage
- 838
- dc.citation.number
- 5
- dc.citation.startPage
- 831
- dc.citation.volume
- 34
- dc.subject.keyword
- Flavonoid
- dc.subject.keyword
- Moleculardocking
- dc.subject.keyword
- Pichiapastoris
- dc.subject.keyword
- Protease
- dc.subject.keyword
- Severeacuterespiratorysyndrome(SARS)
- Appears in Collections:
- 7. KISTI 연구성과 > 학술지 발표논문
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