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공공누리This item is licensed Korea Open Government License

Title
Flavonoid compound-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris
Author(s)
티탄항응우엔안설아
Publication Year
2012-05-01
Abstract
The3C-likeprotease(3CLpro)ofsevereacuterespiratorysyndromeassociatedcoronavirus(SARS-CoV)isvitalforSARS-CoVreplicationandisapromisingdrugtarget.Recombinant3CLprowasexpressedinPichiapastorisGS115asa42kDaproteinthatdisplayedaKmof152withDabcyl-KTSAVLQSGFRKME-Edansassubstrate.Purified3CLprowasusedforinhibitionandkineticassayswithsevenflavonoidcompounds.TheIC50ofsixflavonoidcompoundswere4781.Quercetin,epigallocatechingallateandgallocatechingallate(GCG)displayedgoodinhibitiontoward3CLprowithIC50valuesof73,73and47,respectively.GCGshowedacompetitiveinhibitionpatternwithKivalueof251.7.Inmoleculardockingexperiments,GCGdisplayedabindingenergyof?14kcalmol?1totheactivesiteof3CLproandthegalloylmoietyat3-OHpositionwasrequiredfor3CLproinhibitionactivity.
Keyword
Flavonoid; Moleculardocking; Pichiapastoris; Protease; Severeacuterespiratorysyndrome(SARS)
Journal Title
Biotechnologyletters
Citation Volume
34
ISSN
0141-5492
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Appears in Collections:
7. KISTI 연구성과 > 학술지 발표논문
URI
https://repository.kisti.re.kr/handle/10580/14097
http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART62436871
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