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Title
A signal-on, colorimetric determination of deoxyribonuclease I activity utilizing the photoinduced synthesis of gold nanoparticles
Author(s)
정예림박현규박기수박정훈이창열
Publication Year
2018-03-01
Abstract
simple, colorimetric method is developed for the determination of deoxyribonuclease I (DNase I)
activity based on the novel finding that DNase I can promote the photoinduced synthesis of gold nanoparticles
(AuNPs). In the absence of DNase I, a phosphorothioate (PS) DNA probe remains intact and captures
Au(III) through a strong Au–thiol interaction, which prevents the photoinduced synthesis of AuNPs,
leaving the sample in a colorless state. On the other hand, in the presence of DNase I, the PS DNA probe
is cleaved into small fragments that are removed via a simple purification process. The resulting solution,
after the incubation with HAuCl4 and threonine (Thr), forms AuNPs by UV light irradiation with the aid of
Thr which acts as a catalyst for the Au(III) reduction process. As a result, a red-colored suspension is produced.
By monitoring the color changes of the samples with the naked eye, the DNase I activity was conveniently
determined. In addition, the clinical utility of this simple, yet highly efficient colorimetric strategy
was verified by reliably quantifying the DNase I activities in a bovine urine sample. Importantly, the
working principle designed for the determination of DNase I activity was successfully expanded for the
detection of target nucleic acids, ensuring the universal applicability of the developed assay system.
Journal Title
Nanoscale
Citation Volume
10
ISSN
2040-3364
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Appears in Collections:
7. KISTI 연구성과 > 학술지 발표논문
URI
https://repository.kisti.re.kr/handle/10580/14728
http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART87178776
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