A mass spectrometry-based multiplex SNP genotyping by utilizing allelespecific ligation and strand displacement amplification

Abstract

We herein describe a new mass spectrometry-based method for multiplex SNP genotyping by utilizing allelespecific ligation and strand displacement amplification (SDA) reaction. In this method, allele-specific ligation is first performed to discriminate base sequence variations at the SNP site within the PCR-amplified target DNA. The primary ligation probe is extended by a universal primer annealing site while the secondary