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Title
Influence of Ganglioside GM1 Concentration on Lipid Clustering and Membrane Properties and Curvature
Author(s)
Dhilon S. PatelWonpil ImEmilia L. WuGo¨ran WidmalmJeffery B. KlaudaSoohyung Park염민선
Publication Year
2016-11-01
Abstract
Gangliosides are a class of glycosphingolipids (GSLs) withamphiphilic character that are found at the outer leaflet of the cell membranes, where their ability to organize into special domainsmakes themvital cell membrane components. However, a molecular understanding of GSL-rich membranes in terms of their clustered organization, stability, and dynamics is still elusive. To gain molecular insight into the organization and dynamics of GSL-rich membranes, we performed all-atom molecular-dynamics simulations of bicomponent ganglioside GM1 in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) phospholipid bilayers with varying concentrations ofGM1 (10%, 20%, and 30%).Overall, the simulations showvery good agreement with available experimental data, including x-ray electron density profiles along themembrane normal,NMRcarbohydrate proton-proton distances, and x-ray crystal structures. This validates the quality of our model systems for investigating GM1 clustering through an ordered-lipidcluster analysis. The increase inGM1 concentration induces tighter lipid packing, drivenmainly by inter-GM1 carbohydrate-carbohydrate interactions, leading to a greater preference for the positive curvature of GM1-containing membranes and larger cluster sizes of ordered-lipid clusters (with a composite of GM1 and POPC). These clusters tend to segregate and forma large percolated cluster at a 30% GM1 concentration at 293 K. At a higher temperature of 330 K, however, the segregation is not maintained.
Keyword
Ganglioside GM1 Concentration; Lipid Clustering; Membrane Properties; Membrane Curvature
Journal Title
Biophysical Journal
Citation Volume
111
ISSN
0006-3495
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7. KISTI 연구성과 > 학술지 발표논문
URI
https://repository.kisti.re.kr/handle/10580/14530
http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART76714915
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