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공공누리This item is licensed Korea Open Government License

Title
Establishment of feeder-free culture system for human induced pluripotent stem cell on DAS nanocrystalline graphene
Author(s)
이현아최재경
Publication Year
2016-02-05
Abstract
The maintenance of undifferentiated human pluripotent stem cells (hPSC) under xeno-free condition requires the use of human feeder cells or extracellular matrix (ECM) coating. However, human-derived sources may cause human pathogen contamination by viral or non-viral agents to the patients. Here we demonstrate feeder-free and xeno-free culture system for hPSC expansion using diffusion assisted synthesis-grown nanocrystalline graphene (DAS-NG), a synthetic non-biological nanomaterial which completely rule out the concern of human pathogen contamination. DAS-NG exhibited advanced biocompatibilities including surface nanoroughness, oxygen containing functional groups and hydrophilicity. hPSC cultured on DAS-NG could maintain pluripotency in vitro and in vivo, and especially cell adhesion-related gene expression profile was comparable to those of cultured on feeders, while hPSC cultured without DAS-NG differentiated spontaneously with high expression of somatic cellenriched adhesion genes. This feeder-free and xeno-free culture method using DAS-NG will facilitate the generation of clinical-grade hPSC.
Keyword
graphene scaffold; human pluripotent stem cells (hPSC); human induced pluripotent stem cells (hiPSC); feeder-free culture; diffusion assisted synthesis (DAS); mouse embryonic fibroblast (MEF) feeders
Journal Title
Nature scientific reports
Citation Volume
6
ISSN
2045-2322
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Appears in Collections:
7. KISTI 연구성과 > 학술지 발표논문
URI
https://repository.kisti.re.kr/handle/10580/14497
http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART75128826
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