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공공누리This item is licensed Korea Open Government License

dc.contributor.author
강효진
dc.contributor.author
한기훈
dc.contributor.author
김신현
dc.contributor.author
김윤희
dc.contributor.author
이연금
dc.contributor.author
장인화
dc.contributor.author
진춘매
dc.date.accessioned
2019-08-28T07:42:17Z
dc.date.available
2019-08-28T07:42:17Z
dc.date.issued
2018-11-27
dc.identifier.issn
1756-6606
dc.identifier.uri
https://repository.kisti.re.kr/handle/10580/14765
dc.description.abstract
Various mutations of the SH3 and multiple ankyrin repeat domains 3 (SHANK3) gene, encoding excitatory postsynaptic core scaffolds, are associated with neurodevelopmental and neuropsychiatric disorders. Thus far, synaptic abnormalities in multiple brain regions, including the hippocampus, prefrontal cortex, striatum, and ventral tegmental area, have been investigated in several lines of Shank3 mutant mice. However, even with some reports showing loss and gain of body weight in Shank3 knock-out and overexpressing transgenic (TG) mice, respectively, potential functions of Shank3 in the hypothalamus, a brain region critically involved in energy intake and expenditure, are unknown. To address this issue, we first characterized endogenous Shank3 mRNA and protein expression in the hypothalamus of adult wild-type mice. Then, we performed transcriptome analysis (RNA-sequencing) in the hypothalamus of adult Shank3 TG mice which mildly overexpress Shank3 proteins. By comparing the 174 differentially expressed genes of the hypothalamus with those of previously reported striatum and medial prefrontal cortex (mPFC) in Shank3 TG mice, we found that 159 were hypothalamus-specific while only 15 were shared with either striatum or mPFC. Furthermore, from Gene Set Enrichment Analysis of the RNA-sequencing analysis, we found that ribosome-related genes were enriched especially in the up-regulated genes of Shank3 TG hypothalamus, which is opposite to the analyses on Shank3 TG striatum and mPFC where ribosome-related genes were enriched in the down-regulated genes. Beyond showing endogenous Shank3 mRNA and protein expression in the hypothalamus, our results suggest unique molecular changes in the hypothalamus of Shank3 TG mice compared with the striatum and mPFC.
dc.language
eng
dc.relation.ispartofseries
Molecular Brain
dc.title
Transcriptome analysis of Shank3-overexpressing mice reveals uniquemolecular changes in the hypothalamus
dc.citation.number
71
dc.citation.volume
11
dc.subject.keyword
Shank3
dc.subject.keyword
Hypothalamus
dc.subject.keyword
Transcriptome
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7. KISTI 연구성과 > 학술지 발표논문
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