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공공누리This item is licensed Korea Open Government License

dc.contributor.author
정예림
dc.contributor.author
김문일
dc.contributor.author
김문일
dc.contributor.author
박현규
dc.date.accessioned
2019-08-28T07:41:51Z
dc.date.available
2019-08-28T07:41:51Z
dc.date.issued
2016-02-05
dc.identifier.issn
0957-4484
dc.identifier.uri
https://repository.kisti.re.kr/handle/10580/14500
dc.identifier.uri
http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART76432528
dc.description.abstract
A label-free colorimetric method for the detection of biological thiols (biothiols) was developed.This method is based on prevention of the photoinduced reduction of auric ions (Au(III)) in thepresence of amino acids (acting as a reducing agent) by biothiols; the photoinduced reduction isinhibited due to the strong interaction of the biothiols with Au(III). In this method, the samplewas first incubated in an assay solution containing Au(III) and threonine; the sample solutionwas then exposed to 254 nm UV light. For samples without biothiols, this process led to thephotoreduction of Au(III) followed by growth of gold nanoparticles accompanied by the visuallydetectable development of a red coloration typified by an absorption peak at ca 530 nm.Conversely, in the presence of biothiols, reduction of Au(III) to Au(0) was prevented byentrapment of Au(III) within the biothiols via the thiol group. The solution thus remainedcolorless even after UV irradiation, which was used as an indicator of the presence of biothiols.Using this strategy, biothiols were very conveniently analyzed by monitoring color changes ofthe samples with the naked eye or a UV–vis spectrometer. The strategy based on this interestingphenomenon exhibited high selectivity toward biothiols over common amino acids and wassuccessfully employed for reliable quantification of biothiols present in human plasma,demonstrating its great potential for clinical applications. 
dc.language
eng
dc.relation.ispartofseries
Nanotechnology
dc.title
Label-free colorimetric detection of biological thiols based on target-triggered inhibition of photoinduced formation of AuNPs
dc.citation.number
5
dc.citation.startPage
055501
dc.citation.volume
27
dc.subject.keyword
colorimetric biosensor
dc.subject.keyword
biological thiols
dc.subject.keyword
photoreduction
dc.subject.keyword
gold nanoparticles
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