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공공누리This item is licensed Korea Open Government License

dc.contributor.author
EmiliaL.Wu
dc.contributor.author
염민선
dc.date.accessioned
2019-08-28T07:41:28Z
dc.date.available
2019-08-28T07:41:28Z
dc.date.issued
2014-06-01
dc.identifier.issn
0006-3495
dc.identifier.uri
https://repository.kisti.re.kr/handle/10580/14252
dc.identifier.uri
http://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART69201421
dc.description.abstract
The outer membrane of Gram-negative bacteria is a unique asymmetric lipid bilayer composed of phospholipids (PLs) in the inner leaflet and lipopolysaccharides (LPSs) in the outer leaflet. Its function as a selective barrier is crucial for the survival of bacteria in many distinct environments, and it also renders Gram-negative bacteria more resistant to antibiotics than their Gram-positive counterparts. Here, we report the structural properties of a model of the Escherichia coli outer membrane and its interaction with outer membrane phospholipase A (OmpLA) utilizing molecular dynamics simulations. Our results reveal that given the lipid composition used here, the hydrophobic thickness of the outer membrane is ~3A° thinner than the corresponding PL bilayer, mainly because of the thinner LPS leaflet. Further thinning in the vicinity of OmpLA is observed due to hydrophobic matching. The particular shape of the OmpLA barrel induces various interactions between LPS and PL leaflets, resulting in asymmetric thinning around the protein. The interaction between OmpLA extracellular loops and LPS (headgroups and core oligosaccharides) stabilizes the loop conformation with reduced dynamics, which leads to secondary structure variation and loop displacement compared to that in a DLPC bilayer. In addition, we demonstrate that the LPS/PL ratios in asymmetric bilayers
can be reliably estimated by the per-lipid surface area of each lipid type, and there is no statistical difference in the overall membrane structure for the outer membranes with one more or less LPS in the outer leaflet, although individual lipid properties vary slightly.
dc.language
eng
dc.relation.ispartofseries
Biophysical journal
dc.title
E. coli Outer Membrane and Interactions with OmpLA
dc.subject.keyword
Outer Membrane
dc.subject.keyword
OmpLA
dc.subject.keyword
molecular dynamics simulations
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7. KISTI 연구성과 > 학술지 발표논문
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