Using results from the chiral perturbation theory for the s-wave interaction and the delta-resonance model for the p-wave interaction of pions with nucleons, we have evaluated the spectral functions of pions in asymmetric nuclear matter with unequal proton and neutron densities. We find that in hot dense neutron-rich matter the strength of the spectral function of positively charged pion at low energies is somewhat larger than that of negatively charged pion. In a thermal model, this isospin-de